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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in lots of tumors, which results in chromosome instability, and thus may well add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important part in marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two supplemental splice variants ended up noted but thorough nucleotide sequence information and facts isn't however obtainable.
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Blocking peptides are peptides that bind precisely to your goal antibody and block antibody binding. These peptide commonly includes the epitope acknowledged via the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This manual outlines more doable optimization of mobile and immunoassay parameters to guarantee the very best success are...
Antibodies sure to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which add for the temporal coordination of each mitotic occasion. Cyclin E types a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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